Precision and efficiency

Valita Titer Gen III assay revolutionizes the therapeutic antibody workflow by enabling fast, high-throughput measurement of IgG early in the development process.

Designed for speed, flexibility and ease of use, this cutting-edge assay empowers researchers to make confident, early decisions on lead clones—accelerating the clone selection process and streamlining development timelines.

Combining the reliability of standard methods with enhanced efficiency, this assay delivers high accuracy and precision without compromising on simplicity. Its broad binding properties and increased signal stability support seamless integration and automation across diverse workflows, making it the ideal solution for modern biologics development.

Assay Features 

• New functional range from 0.5 – 100 mg/L*


• Excellent correlation (R² < 0.99) with established methods such as HPLC and BLI


• Increased accuracy, with >96% accuracy and <1.2% relative standard deviation (RSD)


• Binding Human IgG 1,2,4 and rabbit IgG, Fc-fusion proteins, and bispecific antibodies 


• Excellent signal stability up to 55 mins  

Excellent correlation with established methods such as HPLC and BLI, even below 20 mg/L IgG titer

When comparing the Valita Titer Gen III assay with other standard methods to assess IgG titer, such as bio-layer interferometry (BLI) or high-performance liquid chromatography (HPLC) with a protein A column, the resulting data indicated a high correlation of R² > 0.998.  This correlation confirms the high reliability of the Gen III assay when analyzing IgG titer.

Especially in low titer ranges  < 20 mg/L, the Valita Titer Gen III assay shows its high resolution capability with a range of 0.5-100 mg/L. This lets you implement titer quantification early in your clone screening process*.

Valita Titer Gen III Assay vs BLI & HPLC Protein A

Valita Titer Gen III Assay vs BLI (Sub-20mg/L)

Comparison of measured IgG concentration for Valita Titer Gen III assay with HPLC protein A and BLI across 24 samples, ranging from 0.5 - 100 mg/L.

*Accurate quantification is possible from 1.5 mg/L and target detection/ranking from 0.5 mg/L. Quantification and detection limit can be influenced by reagents used (e.g., media) and instrument's inherent performance.

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Increased accuracy for IgG quantification

This new assay provides high accuracy when measuring IgG titer in your samples. With over 96% accuracy it can predict IgG concentration when you measure samples of known concentration. The low standard deviation and coefficient of variation (2.1%) indicate high precision and low variation between measured sample concentrations.

Valita Titer Gen III Assay: Sample Interpolation

Comparison of IgG concentrations measured with Valita Titer Gen III output results. The bar chart shows the average interpolated concentration from 3 replicates, with error bars representing the standard deviation. Average recovery = 98.9 %, average standard deviation = 0.29 mg/L and average %CV = 2.1 across the entire range. 

Versatile detection from human and rabbit IgG to bispecific and Fc-fusion proteins

The Valita Titer Gen III assay probe can detect and quantify standard IgG from human or rabbit species as well as bispecific antibodies (bsAb) through Fc-specific binding. It ensures robust performance with Fc-fusion proteins across a wide range of molecular sizes.

Valita Titer Gen III Assay - Human, Bispecific and Rabbit IgG

The Valita Titer Gen III assay showed comparable results when tested with different antibody types, such as human IgG 1, IgG 2, IgG 4, rabbit pAb and bispecific antibody.

Valita Titer Gen III Assay - Standard IgG vs Fc-Fusion

Measurement of Fc-Fusion proteins of 76kDa or 150kDa is possible with the Valita Titer Gen III assay and gives robust results.

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Excellent signal stability for more than 50 mins

With a signal stability of up to 55 mins after sample addition, the assay offers exceptional flexibility for automation or batch processing, ensuring consistent results without time pressure.

Valita Titer Gen III Assay - Signal Stability

Samples with a known concentration were measured after 5, 15, 25, 35, 45, and 55 minutes incubation time after sample addition and showed a high correlation.

For Research Use Only. Not for use in diagnostic procedures. Valita, Valita Titer, Valita Aggregation Pure and the ValitaCell logo are the trademarks of ValitaCell Ltd in the United States and other countries. ValitaCell is a Beckman Coulter Company.