Resources about Wastewater Research

There has been a lot of research conducted since the beginning of the COVID-19 pandemic. Explore with the links below to learn more about the wastewater research for SARS-COV-2 surveillance:

General resources

• The Water Research Foundation, COVID-19 Guidance and Resources
• The Water Research Foundation, SARS-CoV-2 Wastewater Surveillance Research Coordination Network Kickoff Meeting
• Centers for Disease Control and Prevention, Interim Laboratory Biosafety Guidelines for Handling and Processing Specimens Associated with Coronavirus Disease 2019 (COVID-19)
• Global Water Pathogens Project (GWPP)

Multiple Techniques and General Information 

• SARS-CoV-2 in wastewater: State of the knowledge and research needs. Masaaki Kitajima, Warish Ahmed, Kyle Bibby, Annalaura Carducci, Charles P.Gerba, Kerry A. Hamilton, Eiji Haramoto, Joan B. Rose; Department of Fisheries and Wildlife, Michigan State University, 480 Wilson Road, East Lansing, MI 48824, USA. Posted: April 26, 2020.
• Wastewater monitoring of SARS-CoV-2: lessons from illicit drug policy. Kari Lancaster^a and Tim Rhodesa^b. Posted: June 15, 2020 
  ^aCentre for Social Research in Health, University of New South Wales, Sydney, NSW 2052, Australia
  ^bLondon School of Hygiene and Tropical Medicine, London, UK

Ultracentrifugation

• Sampling and viral concentration for SARS-CoV-2 detection in wastewater. Veronica¹, Antelo, Iraola Lab¹. Posted: May 7, 2020.
  ¹Microbial Genomics Laboratory, Institut Pasteur Montevideo, Uruguay

• Quantification of SARS-CoV-2 and cross-assembly phage from wastewater to monitor coronavirus transmission within communities. Hyatt Green¹*, Maxwell Wilder¹, Mary Collins², Ariana Fenty¹, Karen Gentile³, Brittany L. Kmush⁴, Teng Zeng⁵, Frank A. Middleton³ and David A. Larsen⁴*. Posted: May 23, 2020.
  ¹Department of Environmental and Forest Biology, SUNY-ESF, Syracuse, NY 13210
  ²Department of Environmental Studies, SUNY-ESF, Syracuse, NY 13210
  ³Department of Neuroscience and Physiology, Upstate Medical University, Syracuse, NY 13210
  ⁴Department of Public Health, Syracuse University, Syracuse, NY 13244
  ⁵Department of Civil and Environmental Engineering, Syracuse University, Syracuse, NY 13244

• Detection of rotavirus A in sewage samples using multiplex qPCR and an evaluation of the ultracentrifugation and adsorption-elution methods for virus concentration. Tulio M.Fumian^a, José Paulo G. Leite^a, Alejandro A. Castello^b, Aldo Gaggero^c, Maria Susana L. de Caillou^d, Marize P.Miagostovich^a. Posted: August 30, 2010.
  ^a Laboratory of Comparative and Environmental Virology, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, Brazil
  ^b Laboratorio de Inmunología y Virología, Universidad Nacional de Quilmes Bernal, Argentina
  ^c Programa de Virología, ICBM, Facultad de Medicina, Universidad de Chile, Chile
  ^d Instituto de Microbiología, Universidad de Tucumán, Argentina

• Development of wastewater pooled surveillance of SARS-CoV-2 from congregate living settings. Lisa M. Colosi¹, Katie E. Barry², Shireen M. Kotay², Michael D. Porter¹, Melinda D. Poulter³, Cameron Ratliff⁴, William Simmons⁴, Limor I. Steinberg², D. Derek Wilson⁵, Rena Morse⁶, Paul Zmick⁴ and Amy J. Mathers^2,3*. Posted: October 11, 2020.
  ¹Department of Engineering Systems & Environment, University of Virginia, Charlottesville, VA 22904, USA
  ²Department of Medicine, Division of Infectious Diseases, University of Virginia Health System, Charlottesville, VA 22908, USA
  ³Clinical Microbiology Laboratory, Department of Pathology, University of Virginia Health System, Charlottesville, VA 22908, USA
  ⁴Facilities Management, Energy and Utilities Division, University of Virginia, Charlottesville, VA 22904, USA
  ⁵Facilities Management, University of Virginia Health System, Charlottesville, VA 22908, USA
  ⁶Health Information & Technology, University of Virginia Health System, Charlottesville, Virginia, USA

• Comparison of virus concentration methods for the RT-qPCR-based recovery of murine hepatitis virus, a surrogate for SARS-CoV-2 from untreated wastewater. Warish Ahmed*, Paul M. Bertsch, Aaron Bivins, Kyle Bibby, Kata Farkas, Amy Gathercole, Eiji Haramoto, Pradip Gyawali, Asja Korajkic, Brian R. McMinn, Jochen F. Mueller, Stuart L. Simpson, Wendy J.M. Smith, Erin M. Symonds, Kevin V. Thomas, Rory Verhagen, and Masaaki Kitajimal. Posted: October 15, 2020.
  *Corresponding author at: Ecosciences Precinct, 41 Boggo Road, Dutton Park 4102, Queensland, Australia. For additional authors affiliations, please refer to the author information in the article.  

• The efficiency of concentration methods used to detect enteric viruses in anaerobically digested sludge. Tatiana Prado^1*, Wilma de Carvalho Pereira Bonet Guilayn², Ana Maria Coimbra Gaspar³, and Marize Pereira Miagostovich¹. Posted: Feb 2013.
  ¹Laboratório de Virologia Comparada e Ambiental
  ²Departamento de Saneamento e Saúde Ambiental, Escola Nacional de Saúde Pública-Fiocruz, Rio de Janeiro, RJ, Brasil
  ³Laboratório de Desenvolvimento Tecnológico em Virologia, Instituto Oswaldo Cruz-Fiocruz, Rio de Janeiro, RJ, Brasil

Ultrafiltration

• Correlation of SARS-CoV-2 RNA in wastewater with COVID-19 disease burden in sewersheds (Utah study). Jennifer Weidhaas^a⁎, Zachary T. Aanderud^b, D. Keith Roper^c, James VanDerslice^d, Erica Brown Gaddis^e, Jeff Ostermiller^e, Ken Hoffman^e, Rubayat Jamal^a, Phillip Heck^f, Yue Zhang^g, Kevin Torgersen^b, Jacob Vander Laan^e, and Nathan LaCross^h. Posted: June 25, 2020.
  ^aUniversity of Utah, Civil and Environmental Engineering, 110 Central Campus Drive, Suite 2000, Salt Lake City, UT, USA
  ^bBrigham Young University, Department of Plant & Wildlife Sciences, Provo, UT, USA
  ^cUtah State University, Biological Engineering, 4105 Old Main Hill, Logan, UT, USA
  ^dUniversity of Utah, Family and Preventive Medicine, 275 Chipeta Way, Suite A, Salt Lake City, UT, USA
  ^eUtah Department of Environmental Quality, Division of Water Quality, 195 N 1950 West, Salt Lake City, UT, USA
  ^fCentral Valley Water Reclamation Facility, 800 West Central Valley Road, Salt Lake City, UT, USA
  ^gUniversity of Utah, School of Medicine, Department of Internal Medicine, Division of Epidemiology, 295 South Chipeta Way, Salt Lake City, UT, USA
  ^hUtah Department of Health, Division of Disease Control and Prevention, Bureau of Epidemiology, 288 N 1460 W, Salt Lake City, UT, USA

• First environmental surveillance for the presence of SARS-CoV-2 RNA in wastewater and river water in Japan. Eiji Haramoto^a, Bikash Malla^a Ocean Thakali^b, Masaaki Kitajima^c. Posted: October 1, 2020.
  ^aInterdisciplinary Center for River Basin Environment, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi 400-8511, Japan
  ^bEnvironmental and Social System Science Course, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi 400-8511, Japan
  ^cDivision of Environmental Engineering, Hokkaido University, North 13 West 8, Kita-ku, Sapporo, Hokkaido 060-8628, Japan

• The wastewater microbiome: A novel insight for COVID-19 surveillance. Cristian Gallardo-Escárate, Valentina Valenzuela-Muñoz^ab, Gustavo Núñez-Acuña^ab, Diego Valenzuela-Miranda^ab, Bárbara P.Benaventel, Constanza Sáez-Vera, Homero Urrutia, Beatriz Novoa, Antonio Figueras, Steven Roberts, Paulina Assmann, Marta Bravo. Posted: October 9, 2020.
  ^a Interdisciplinary Center for Aquaculture Research, University of Concepción, O'Higgins 1695, Concepción, Chile
  ^b Laboratory of Biotechnology and Aquatic Genomics, Center of Biotechnology, Barrio   Universitario s/n, University of Concepción, Concepción, Chile
  For additional authors affiliations, please refer to the author information in the article. 

• Standard Operating Procedure for Sampling, Concentration and Detection of SARS-CoV-2 in Sewage published by Joan Rose, PhD, et al. Matthew Flood, Nishita D’Souza, Tiong Gim, and Joan Rose. Posted: October 17, 2020.

• First detection of SARS-CoV-2 RNA in wastewater in North America: A study in Louisiana, USA. Samendra P.Sherchan^a, Shalina Shahin^a, Lauren M. Ward^a, Sarmila Tandukar^b, Tiong G. Aw^a, Bradley Schmitz^c, Warish Ahmed^d, Masaaki Kitajima^e. Posted: November 15, 2020.
  ^aDepartment of Environmental Health Sciences, Tulane University, 1440 Canal Street, Suite 2100, New Orleans, LA 70112, USA
  ^bInterdisciplinary Center for River Basin Environment, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi 400-8511, Japan
  ^cLoudoun Water, 44865 Loudoun Water Way, Ashburn, VA 20147, USA
  ^dCSIRO Land and Water, Ecosciences Precinct, 41 Boggo Road, Dutton Park, QLD 4102, Australia
  ^eDivision of Environmental Engineering, Faculty of Engineering, Hokkaido University, North 13 West 8, Kita-ku, Sapporo, Hokkaido 060-8628, Japan

Chemical/Biochemical Precipitation

• Metropolitan wastewater analysis for COVID-19 epidemiological surveillance. Walter Randazzo^ab, Enric Cuevas-Ferrando^a, Rafael Sanjuán^c, Pilar Domingo-Calap^cd, Gloria Sánchez^a. Posted: September 1, 2020
  ^a Department of Preservation and Food Safety Technologies, Instituto de Agroquímica y Tecnología de Alimentos – Consejo Superior de Investigaciones Científicas (IATA-CSIC), Av. Agustín Escardino 7, Paterna, 46980, Valencia, Spain
  ^b Department of Microbiology and Ecology, Universitat de València, Av. Dr. Moliner, 50, Burjassot, 46100, Valencia, Spain
  ^c Institute for Integrative Systems Biology, I2SysBio, Universitat de València-CSIC, 46980, Paterna, Spain
  ^d Department of Genetics, Universitat de València, 46980, Paterna, Spain

   

*Corresponding author