The SuperNova Staining Buffer has been designed to avoid non-specific interactions between SuperNova antibody conjugates when they are mixed in a cocktail for a flow cytometry experiment. The SuperNova Staining Buffer is only necessary when there is more than one SuperNova antibody conjugate in the cocktail. Abnormal stainings can occur in case of absence or misuse of the SuperNova Staining Buffer (data can appear under-compensated). The SuperNova Staining Buffer is compatible with the use of other fluorochrome antibody conjugates, with a surface or intracellular procedure.