EMnetik Plasmid Purification System Performance Data
Plasmid Prep - Simplified
- Plasmid recovery of 4-7 µg shown for a high copy plasmid
- No need to handle small columns or use a single-channel pipette
- Move samples from the lysate to the EMnetik 24 system, and don’t move them again until final elution
- Intuitive user interface removes guesswork by providing clear, step-by-step instructions
Choose your sample input, elution and labware.
Figure 1
Figure 1 left: Yield of elutions from 2 different elution volume options (50 µL and 20 µL). The bars are the average of 8 replicates on the device, and the error bars are the standard deviation of the replicates. The left middle and right refer to the placement in the EMnetik 24 system; for example, the left most column of samples are averaged in the left grey bars. Figure 1 right: Yield does not vary widely when using different labware. The first three bars show the yield using PCR 8-strip tubes, and the second three bars show the yield using single PCR tubes. The bars represent the average of 8 samples; error bars indicate the standard deviation of the 8 samples. Tubes are as follows: A: Thermo Scientific AB-2005, B:VWR 93001-118, C: VWR 20170-002, D: Thermo Scientific AB-0337, E: VWR 20170-010, and F: VWR 20170-012
Plasmid extraction is comparable to manual cleanup protocols.
Figure 2
Figure 2: The concentration of pUC19 plasmid using EMnetik 24 and a manual bead-based chemistry were compared post extraction. The manual comparison bar is an average of 3 replicates, and the EMnetik bars are an average of 8 replicates. The error bars are the standard deviation.
EMnetik Plasmid Purification Workflow
EMnetik Plasmid Purification Workflow
- Pellet your sample
- Add L1 to lyse your sample
- Add N3 to neutralize your sample
- Pellet the flocculant and remove the supernatant to a new tube
- Add your sample to the instrument
- Add your EMnetik plasmid purification bind to your sample
- Start bind mix and separate
- Remove supernatant
- Add ethanol to wash
- Remove supernatant
- Repeat steps 5 and 6
- Start your ethanol dry
- Add your eluant
- Start elution mix and separate
- Move your final elution to your preferred labware
Not intended or validated for use in the diagnosis of disease or other conditions.
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