The Complexities of SV Analytical Ultracentrifugation: AAVs are not Simple, Binary Systems
Speaker: Dr. Lake Paul - Founder and President, BioAnalysis, LLC
Date: September 3, 2020
Abstract
As SV-AUC becomes the go-to method for quantitation and characterization of AAV, caution must be taken during the experimental design and analysis. The heterogeneous nature of the intermediate capsids, possible self-associations, pressure effect of the hydrodynamic shape of the capsids, and even the approach to the data fitting are factors that must be carefully considered. A complete and robust understanding of the aforementioned factors along with a strong experimental design can produce reliable SV-AUC methods suitable for cGMP qualification and beyond
Learning Objectives
- Identify approaches to correctly analyzing AAV systems.
- Applying Analytical Ultracentrifugation (AUC) in a cGMP environment.
- Develop proper experimental design for AAV systems and extracting the correct information.
