We’re excited to share our key findings and insights with you

We collaborated with BridgeBio to demonstrate a simultaneous DNA and RNA extraction method that doesn’t require splitting the sample lysate. This method has shown consistent performance across species with 10 different tissue types and 9 regions of the brain. It uses our SPRI bead-based technology that selectively binds DNA and RNA to the beads, enabling independent separation.

Here’s Why We Did This

As you know, in the preclinical study phase, vector copy number (VCN) and transgene expression are often tested from the same sample to ensure the accuracy and reproducibility of treatment performance. To do so, both DNA and RNA are extracted from the same biologic sample.

Some existing protocols achieve simultaneous DNA and RNA extraction by splitting the lysate into two portions, then treating the portions of lysate with either DNase or RNase A. The drawback of this method, however, especially for precious samples, is that half of the DNA or RNA is lost.